3DD7
Structure of DocH66Y in complex with the C-terminal domain of Phd
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9189, 0.9116 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 110.944, 38.201, 63.734 |
Unit cell angles | 90.00, 99.26, 90.00 |
Refinement procedure
Resolution | 11.000 - 1.700 |
R-factor | 0.18317 |
Rwork | 0.182 |
R-free | 0.19849 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.237 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 11.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.090 | 0.950 |
Number of reflections | 29371 | |
<I/σ(I)> | 9 | 2 |
Redundancy | 7.1 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 35% PEG4000, 0.2M ammonium acetate, 0.1M acetate, pH4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |