3DD4
Structural Basis of KChIP4a Modulation of Kv4.3 Slow Inactivation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-08-25 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | I 41 |
Unit cell lengths | 96.300, 96.300, 71.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.2392 |
Rwork | 0.239 |
R-free | 0.29880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | KChIP1 (PDB code: 2NZ0) |
RMSD bond length | 0.008 |
RMSD bond angle | 1.210 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.058 | 0.567 |
Number of reflections | 6650 | |
<I/σ(I)> | 37.9 | 4.1 |
Completeness [%] | 99.6 | 100 |
Redundancy | 8 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.6-1.8M Ammonium Sulfate, 4%(v/v) iso-Propanol, 0.1M DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |