3DCP
Crystal structure of the putative histidinol phosphatase hisK from Listeria monocytogenes. Northeast Structural Genomics Consortium target LmR141.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-21 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97931 |
Spacegroup name | P 64 |
Unit cell lengths | 191.278, 191.278, 48.051 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.560 - 2.100 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.21200 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXDE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | |
Rmerge | 0.106 | 0.272 |
Number of reflections | 114574 | |
<I/σ(I)> | 23.6 | 4.4 |
Completeness [%] | 99.8 | 98.4 |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 15% PEG 8000, 0.17 M sodium acetate, 0.01 M L-cysteine, 0.1 M MES pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |