3DCB
Crystal structure of the Drosophila kinesin family member NOD in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Detector technology | CCD |
| Collection date | 2007-11-17 |
| Detector | ADSC |
| Wavelength(s) | 0.9781 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.864, 74.780, 79.956 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.000 - 2.500 |
| Rwork | 0.209 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dc4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.600 |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 2.700 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.102 | 0.450 |
| Number of reflections | 10899 | 2224 |
| <I/σ(I)> | 15.69 | 4.4 |
| Completeness [%] | 96.9 | 98.5 |
| Redundancy | 4.7 | 4.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 MES, 15% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
