3DC7
Crystal structure of the protein Q88SR8 from Lactobacillus plantarum. Northeast Structural Genomics consortium target LpR109.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-22 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9791, 0.9679, 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.610, 101.440, 125.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.740 - 2.120 |
| R-factor | 0.18598 |
| Rwork | 0.184 |
| R-free | 0.22431 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.120 | 2.270 |
| Rmerge | 0.078 | 0.229 |
| Number of reflections | 54518 | |
| <I/σ(I)> | 20.4 | 7.5 |
| Completeness [%] | 99.1 | 97.5 |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M MgSo4, 0.1M Bis-Tris, 16% PEG8K, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
