3DC4

Crystal structure of the Drosophila kinesin family member NOD in complex with ADP

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Detector technology	CCD
Collection date	2007-04-07
Detector	ADSC
Wavelength(s)	0.9790, 0.9796, 0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	47.829, 75.715, 94.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.000 - 1.900
Rwork	0.227
R-free	0.25500
Structure solution method	MAD, MR
RMSD bond length	0.006
RMSD bond angle	1.600
Data scaling software	XSCALE
Phasing software	SOLVE (2.13)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.057	0.342
Number of reflections	27088	3809
<I/σ(I)>	22.77	4.9
Completeness [%]	98.6	98.8
Redundancy	4.81	4.88

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1 MES, 15% PEG 20000, Se-MET Derivative, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1 MES, 15% PEG 20000, Se-MET Derivative, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K