3DBY
Crystal structure of uncharacterized protein from Bacillus cereus G9241 (CSAP Target)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | H 3 |
Unit cell lengths | 167.808, 167.808, 582.661 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.201 |
Rwork | 0.198 |
R-free | 0.25800 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.540 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.161 | 0.790 |
Number of reflections | 346752 | |
<I/σ(I)> | 10.7 | 1.9 |
Completeness [%] | 97.0 | 94.9 |
Redundancy | 4.8 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 24% PEG 3350, 0.2M Lithium acetate, Vapor diffusion, Sitting drop, temperature 298K |