3DBJ
Allophycocyanin from Thermosynechococcus vulcanus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 3 |
| Unit cell lengths | 102.597, 102.597, 128.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| Rwork | 0.212 |
| R-free | 0.26950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1all |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.410 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.091 | 0.248 |
| Number of reflections | 30684 | |
| <I/σ(I)> | 9.1 | 3.7 |
| Completeness [%] | 93.0 | 88.8 |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 6% PEG 4000, 400mM MgSO4, 20mM Tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
