3DB5

Crystal structure of methyltransferase domain of human PR domain-containing protein 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-05-24
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54178
Spacegroup name	I 4 2 2
Unit cell lengths	107.294, 107.294, 133.642
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.226
Rwork	0.222
R-free	0.29800
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	1.926
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.230
High resolution limit [Å]	2.150	4.630	2.150
Rmerge	0.093	0.048	0.799
Number of reflections	21499
<I/σ(I)>	13.1
Completeness [%]	100.0	100	100
Redundancy	27.2	26.8	18.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1.5 microliter protein solution, 1.5 microliter reservoir solution containing 23% PEG 3350, 0.2 M Ammonium acetate, 0.1 M Bis-Tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K