3DB5
Crystal structure of methyltransferase domain of human PR domain-containing protein 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E DW |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-05-24 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54178 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 107.294, 107.294, 133.642 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.150 |
R-factor | 0.226 |
Rwork | 0.222 |
R-free | 0.29800 |
Structure solution method | SAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.926 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.230 |
High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
Rmerge | 0.093 | 0.048 | 0.799 |
Number of reflections | 21499 | ||
<I/σ(I)> | 13.1 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 27.2 | 26.8 | 18.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.5 microliter protein solution, 1.5 microliter reservoir solution containing 23% PEG 3350, 0.2 M Ammonium acetate, 0.1 M Bis-Tris pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |