3DAW

Structure of the actin-depolymerizing factor homology domain in complex with actin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-29
Detector	ADSC QUANTUM 315
Wavelength(s)	1.072250
Spacegroup name	P 21 21 21
Unit cell lengths	52.780, 72.990, 168.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.760 - 2.550
R-factor	0.21199
Rwork	0.208
R-free	0.27925
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hd7 2a42
RMSD bond length	0.012
RMSD bond angle	1.534
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.610
High resolution limit [Å]	2.550	2.550
Rmerge	0.131	0.552
Number of reflections	26755
<I/σ(I)>	9.6	2.3
Completeness [%]	99.3	99.8
Redundancy		3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10mM Tris pH 7.5, 50mM NaCl, 0.2mM ATP, 0.2mM DTT, 0.2mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K