3DAV
Schizosaccharomyces Pombe Profilin crystallized from Sodium formate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 273 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.873, 82.426, 39.742 |
| Unit cell angles | 90.00, 92.59, 90.00 |
Refinement procedure
| Resolution | 41.200 - 2.200 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.26000 |
| Starting model (for MR) | 3d9y |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.146 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.318 |
| Number of reflections | 11429 | |
| <I/σ(I)> | 15.28 | 4.03 |
| Completeness [%] | 95.8 | 64 |
| Redundancy | 2.9 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296.15 | 4.0 M sodium formate , VAPOR DIFFUSION, HANGING DROP, temperature 296.15K |
