3DA4
Crystal Structure of Colicin M, a Novel Phosphatase Specifically Imported by Escherichia Coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.490, 108.750, 224.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.22136 |
| Rwork | 0.218 |
| R-free | 0.26134 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3da3 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.771 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.123 | 0.452 |
| Number of reflections | 67044 | |
| <I/σ(I)> | 9.9 | 2.4 |
| Completeness [%] | 97.7 | 93.8 |
| Redundancy | 5.6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.1M N-(2-acetamido) iminodiacetate, 12% PEG 6000, 0.2M MgCl2, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
