3DA3
Crystal Structure of Colicin M, A Novel Phosphatase Specifically Imported by Escherichia Coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 119.880, 119.880, 96.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.500 |
| R-factor | 0.23802 |
| Rwork | 0.235 |
| R-free | 0.29468 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.252 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.175 | 0.398 |
| Number of reflections | 23282 | |
| <I/σ(I)> | 8 | 2.7 |
| Completeness [%] | 93.2 | 98 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 282 | 20% PEG 3350, 0.2M potassium nitrate, 0.1mM CaCl2, 0.1mM pyrophosphate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 282K |
