3DA0
Crystal structure of a cleaved form of a chimeric receptor binding protein from Lactococcal phages subspecies TP901-1 and p2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9340 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.168, 43.682, 78.946 |
| Unit cell angles | 90.00, 101.63, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.650 |
| R-factor | 0.16505 |
| Rwork | 0.164 |
| R-free | 0.20149 |
| Structure solution method | MIR |
| Starting model (for MR) | 2bsd |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.686 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.010 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.166 | 0.394 |
| Number of reflections | 48882 | |
| <I/σ(I)> | 7.7 | 2.3 |
| Completeness [%] | 98.3 | 99.9 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 293 | 0.1M Imidazole-malate, 17% PEG4000, pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
