3D9S

Human Aquaporin 5 (AQP5) - High Resolution X-ray Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	170
Detector technology	CCD
Collection date	2007-07-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.931
Spacegroup name	P 21 21 21
Unit cell lengths	90.477, 90.644, 184.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
Rwork	0.162
R-free	0.19300
Structure solution method	AB INITIO
Starting model (for MR)	1j4n
RMSD bond length	0.006
RMSD bond angle	0.024
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.560	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	84751
<I/σ(I)>	8.3	1.5
Completeness [%]	82.9	82.6
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	281	19% polyethylene glycol 400, 0.1M Tris HCl pH 7.6, 0.1M NaCl, 6% v/v 1,6-hexanediol, 3% v/v 1,4-butanediol or 3% v/v 1,3-propanediol, VAPOR DIFFUSION, HANGING DROP, temperature 281K