3D9P

Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the Carboxy-Terminal Domain of RNA-Polymerase II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9782
Spacegroup name	P 43
Unit cell lengths	57.520, 57.520, 106.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.620 - 2.100
R-factor	0.18357
Rwork	0.181
R-free	0.23496
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3d9j
RMSD bond length	0.011
RMSD bond angle	1.111
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC (5.2.0019)
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	20039
<I/σ(I)>	21.6	5.9
Completeness [%]	98.8	97.6
Redundancy	5.1	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.2 M Li2SO4, 1.6 M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K