3D9J
Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the Carboxy-Terminal Domain of RNA-Polymerase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0007 |
| Spacegroup name | P 43 |
| Unit cell lengths | 57.620, 57.620, 107.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.760 - 1.600 |
| R-factor | 0.17987 |
| Rwork | 0.179 |
| R-free | 0.20568 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3d9i |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.366 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC (5.2.0019) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 44703 | |
| <I/σ(I)> | 24 | 6.1 |
| Completeness [%] | 97.3 | 94.3 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M citric acid, 2.8 M (NH4)2SO4, 1% (v/v) glycerol , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
