3D96
Crystal Structure of the R132K:Y134F Mutant of Apo-Cellular Retinoic Acid Binding Protein Type II at 1.71 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2002-11-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.590, 37.230, 58.770 |
| Unit cell angles | 73.90, 74.82, 87.90 |
Refinement procedure
| Resolution | 50.000 - 1.710 |
| R-factor | 0.16 |
| Rwork | 0.153 |
| R-free | 0.22200 |
| Structure solution method | RIGID BODY REFINEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.598 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.2.0005) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.052 | 0.240 |
| Number of reflections | 26617 | |
| <I/σ(I)> | 20.44 | 1.55 |
| Completeness [%] | 89.7 | 48.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% (W/V) PEG 4000, 0.1 M TRIS-HCL, 0.2 M SODIUM ACETATE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
