3D95
Crystal Structure of the R132K:Y134F:R111L:L121E:T54V Mutant of Apo-Cellular Retinoic Acid Binding Protein Type II at 1.20 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2004-03-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.591, 36.897, 57.811 |
| Unit cell angles | 73.28, 75.91, 87.76 |
Refinement procedure
| Resolution | 40.000 - 1.200 |
| R-factor | 0.141 |
| Rwork | 0.138 |
| R-free | 0.17600 |
| Structure solution method | RIGID BODY REFINEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.676 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.2.0005) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.066 | 0.271 |
| Number of reflections | 77347 | |
| <I/σ(I)> | 18.17 | 2.55 |
| Completeness [%] | 93.8 | 89.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 20% (W/V) PEG 6000, 0.1 M SODIUM ACETATE, 5% ETHANOL, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
