3D8L
Crystal structure of ORF12 from the lactococcus lactis bacteriophage p2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9785 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 158.318, 158.318, 99.524 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.20503 |
| Rwork | 0.203 |
| R-free | 0.25319 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.591 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.092 | 0.092 |
| Number of reflections | 10739 | |
| <I/σ(I)> | 29.1 | 6.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 21.4 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 0.1M HEPES, 15.9% PEG600, pH6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
