3D8H
Crystal structure of phosphoglycerate mutase from Cryptosporidium parvum, cgd7_4270
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.248, 158.365, 140.249 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.500 - 2.010 |
| R-factor | 0.233 |
| Rwork | 0.231 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xq9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.141 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.075 | 0.796 |
| Number of reflections | 39125 | |
| <I/σ(I)> | 9.6 | 2.32 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 273 | 25% PEG 3350, 0.2 M NH4OAc, 0.1 M Hepes pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
