3D8G
Crystal structure of Staphylococcal nuclease variant Delta+PHS I72R at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-13 |
| Detector | BRUKER APEX II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.181, 60.425, 73.124 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 1.990 - 1.990 |
| R-factor | 0.205 |
| Rwork | 0.200 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RCSB entry 1tqo all waters removed b = 20.0 ILE-72 and ILE-92 truncated to ALA |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.226 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 2.090 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.021 | 0.097 |
| Number of reflections | 10279 | |
| <I/σ(I)> | 42.54 | 9.87 |
| Completeness [%] | 99.7 | 97.6 |
| Redundancy | 20.75 | 9.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTP, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
