3D8F
Crystal structure of the human Fe65-PTB1 domain with bound phosphate (trigonal crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | H 3 |
| Unit cell lengths | 146.040, 146.040, 78.294 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.210 - 2.700 |
| R-factor | 0.245 |
| Rwork | 0.242 |
| R-free | 0.31300 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.635 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.077 | 0.341 |
| Number of reflections | 17113 | |
| <I/σ(I)> | 12.9 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 100mM HEPES, 5% (v/v) ethylene glycol, 50mM Na2HPO4, 10% (w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, temperature 291K |
