3D8E
Crystal structure of the human Fe65-PTB1 domain (trigonal crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0723 |
| Spacegroup name | H 3 |
| Unit cell lengths | 145.890, 145.890, 79.051 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.800 |
| R-factor | 0.246 |
| Rwork | 0.240 |
| R-free | 0.30400 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.776 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.900 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.089 | 0.347 |
| Number of reflections | 15431 | |
| <I/σ(I)> | 14.2 | 3.2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 100mM HEPES, 5% (v/v) ethylene glycol, 10% (w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, temperature 291K |
