3D8D
Crystal structure of the human Fe65-PTB1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0073 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.756, 79.574, 83.974 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.000 - 2.200 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.25200 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.445 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.900 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 | 0.347 |
| Number of reflections | 15508 | |
| <I/σ(I)> | 19.2 | 4.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 100mM HEPES, 5% (v/v) ethylene glycol, 8% (w/v) PEG 3350, 1mM CH3HgCl, pH 7.5, VAPOR DIFFUSION, temperature 291K |
