3D8B
Crystal structure of human fidgetin-like protein 1 in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 85.430, 85.430, 197.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.990 - 2.000 |
| R-factor | 0.19909 |
| Rwork | 0.197 |
| R-free | 0.23669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b9p |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.421 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 | 0.556 |
| Number of reflections | 49968 | |
| <I/σ(I)> | 20 | 5.2 |
| Completeness [%] | 99.3 | 99.6 |
| Redundancy | 7.8 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25% PEG 3350, 0.2M NaCl, 0.1M Bis-Tris, 0.02M ADP, 0.01M MgCl2, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
