3D89
Crystal Structure of a Soluble Rieske Ferredoxin from Mus musculus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.96419 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 52.413, 52.413, 108.808 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.750 - 2.071 |
R-factor | 0.2 |
Rwork | 0.199 |
R-free | 0.22800 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.848 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.070 | 4.460 | 2.070 |
Rmerge | 0.057 | 0.038 | 0.621 |
Number of reflections | 9806 | ||
<I/σ(I)> | 18.354 | 1.845 | |
Completeness [%] | 99.4 | 98.7 | 95.5 |
Redundancy | 12.7 | 12.3 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | Protein Solution (~15 mg/ml native protein, 0.025 M NaCl, 0.005 M MOPS pH 7.0) mixed in a 1:1 ratio with Well Solution ( 25% MEPEG 5K, 0.12 M Trisodium citrate, 0.10 M MES ph 6.0) Cryoprotected with 15% ethylene glycol; Mercury derivative crystal soaked overnight in in mother liquor containing 0.002 M thimersol, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | Protein Solution (~15 mg/ml native protein, 0.025 M NaCl, 0.005 M MOPS pH 7.0) mixed in a 1:1 ratio with Well Solution ( 25% MEPEG 5K, 0.12 M Trisodium citrate, 0.10 M MES ph 6.0) Cryoprotected with 15% ethylene glycol; Mercury derivative crystal soaked overnight in in mother liquor containing 0.002 M thimersol, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP |