3D7Q
Crystal structure of a xisi-like protein (npun_ar114) from nostoc punctiforme pcc 73102 at 2.30 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-13 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97937,0.97854 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.846, 79.846, 74.256 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.228 - 2.300 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.25900 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.487 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.228 | 28.230 | 2.360 |
High resolution limit [Å] | 2.300 | 10.290 | 2.300 |
Rmerge | 0.088 | 0.047 | 0.766 |
Total number of observations | 794 | 5800 | |
Number of reflections | 11187 | ||
<I/σ(I)> | 6.5 | 11.6 | 1 |
Completeness [%] | 99.9 | 95 | 100 |
Redundancy | 7 | 5.2 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 0.15M lithium sulfate, 26.0% polyethylene glycol 4000, 0.1M TRIS pH 9.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |