3D7P
Crystal structure of human Transthyretin (TTR) at pH 4.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-10 |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.224, 85.082, 62.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.190 - 1.720 |
| R-factor | 0.22276 |
| Rwork | 0.221 |
| R-free | 0.24943 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bmz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.353 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.190 | 1.760 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Number of reflections | 25276 | |
| Completeness [%] | 99.7 | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 289 | TTR pH 4.0 crystals were grown in the presence of 0.1 M sodium acetate (pH 4.0), 100 mM KCl, 1 mM EDTA and 0.3-0.5 M ammonium sulfate which was equilibrated against 2 M ammonium sulfate in hanging drop, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
