3D7N
The crystal structure of the flavodoxin, WrbA-like protein from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-22 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 73.948, 73.948, 144.488 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.520 - 2.300 |
| R-factor | 0.20954 |
| Rwork | 0.207 |
| R-free | 0.25378 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.766 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.520 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.097 | 0.650 |
| Number of reflections | 10224 | |
| <I/σ(I)> | 35 | 1.45 |
| Completeness [%] | 97.6 | 92.32 |
| Redundancy | 24.7 | 17.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Hepes, 0.2M NaCl, 25%PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
