3D79
Crystal structure of hypothetical protein PH0734.1 from hyperthermophilic archaea Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 52.921, 52.921, 133.313 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 1.730 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.126 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 1.790 |
High resolution limit [Å] | 1.730 | 3.720 | 1.730 |
Rmerge | 0.065 | 0.055 | 0.221 |
Number of reflections | 23373 | ||
<I/σ(I)> | 21.3 | ||
Completeness [%] | 99.8 | 98.7 | 100 |
Redundancy | 18.8 | 18.4 | 19 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |