3D78
Dimeric crystal structure of a pheromone binding protein mutant D35N, from apis mellifera, at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.054, 60.948, 57.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.600 |
| R-factor | 0.16088 |
| Rwork | 0.159 |
| R-free | 0.20597 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3d73 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.498 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC (5.2.0019) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.048 | 0.321 |
| Number of reflections | 32103 | |
| <I/σ(I)> | 20.3 | 3.8 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.1M ammonium sulfate, 0.2M di ammonium phosphate, 20mM di sodium phosphate, pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
