3D77
Crystal structure of a pheromone binding protein mutant D35N, from Apis mellifera, soaked at pH 4.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 79.215, 83.999, 47.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
R-factor | 0.16309 |
Rwork | 0.161 |
R-free | 0.19131 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3d75 |
RMSD bond length | 0.014 |
RMSD bond angle | 2.211 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC (5.2.0019) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.640 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.047 | 0.349 |
Number of reflections | 17650 | |
<I/σ(I)> | 26.4 | 4.5 |
Completeness [%] | 99.1 | 99.6 |
Redundancy | 6.8 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 1.3M ammonium sulfate, 67mM sodium citrate, 33mM SPG buffer, 8.3% PEG1500, pH5.5, crystal was soaked in the same condition at pH4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |