3D76
Crystal structure of a pheromone binding protein mutant D35N, from Apis mellifera, soaked at pH 7.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-01-22 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.542 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 78.991, 84.196, 47.558 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.900 |
R-factor | 0.19423 |
Rwork | 0.190 |
R-free | 0.24146 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3d75 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.848 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC (5.2.0019) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.130 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.056 | 0.322 |
Number of reflections | 12833 | |
<I/σ(I)> | 24.8 | 6.5 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6.1 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.3M ammonium sulfate, 67mM sodium citrate, 33mM SPG buffer, 8.3% PEG1500, pH5.5, crystal was soaked in the same condition at pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |