3D76
Crystal structure of a pheromone binding protein mutant D35N, from Apis mellifera, soaked at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.542 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.991, 84.196, 47.558 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.19423 |
| Rwork | 0.190 |
| R-free | 0.24146 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3d75 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.848 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC (5.2.0019) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.130 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.056 | 0.322 |
| Number of reflections | 12833 | |
| <I/σ(I)> | 24.8 | 6.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.3M ammonium sulfate, 67mM sodium citrate, 33mM SPG buffer, 8.3% PEG1500, pH5.5, crystal was soaked in the same condition at pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
