3D6O
The RNase A- 5'-Deoxy-5'-N-(ethyl isonipecotatyl)uridine complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.92 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.384, 32.876, 72.621 |
Unit cell angles | 90.00, 90.40, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.580 |
R-factor | 0.18793 |
Rwork | 0.186 |
R-free | 0.22678 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2g8q |
RMSD bond length | 0.009 |
RMSD bond angle | 1.155 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC (5.3) |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.620 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.057 | |
Number of reflections | 31074 | |
<I/σ(I)> | 16.5 | 3.8 |
Completeness [%] | 94.9 | 96.6 |
Redundancy | 7.3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | PEG 4000, Sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |