3D5L
Crystal structure of regulatory protein RecX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.190, 81.924, 124.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.590 - 2.350 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.30000 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.080 | 0.220 |
| Number of reflections | 23443 | |
| <I/σ(I)> | 9.5 | 1 |
| Completeness [%] | 92.4 | 55.6 |
| Redundancy | 6.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 200mM Ammonium sulfate, 25% PEG 3350, 100mM Bis-tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
