3D4Z
GOLGI MANNOSIDASE II complex with gluco-imidazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-20 |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.649, 109.303, 137.762 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.390 |
| Rwork | 0.144 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.027 |
| Data reduction software | HKL-2000 |
| Data scaling software | SADABS |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.410 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.061 | 0.235 |
| Number of reflections | 207007 | |
| <I/σ(I)> | 19.4 | 5.9 |
| Completeness [%] | 99.5 | 97.5 |
| Redundancy | 7.03 | 5.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | PEG8000, Tris, pH 7, 2.5% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.00 |
