3D4W
Crystal structure of Staphylococcal nuclease variant Delta+PHS A109R at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-10 |
| Detector | BRUKER APEX II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.090, 59.843, 73.558 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.300 - 1.900 |
| R-factor | 0.197 |
| Rwork | 0.191 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tqo all waters removed b = 20.0 ILE-92 truncated to ALA |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.430 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.100 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.020 | 0.108 |
| Number of reflections | 12416 | |
| <I/σ(I)> | 41.54 | 8.46 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 12.05 | 5.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 43% MPD, 25 mM Potassium Phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
