3D4M
Glutaredoxin 2 oxidized structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-07-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.431 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 47.634, 47.634, 94.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.560 - 2.050 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.418 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.560 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 7295 | |
| <I/σ(I)> | 6.3 | 7.8 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 12.3 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | PEG 4000, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
