3D4D
Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-02-06 |
| Detector | BRUKER APEX II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.022, 81.293, 60.385 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 60.380 - 2.100 |
| R-factor | 0.239 |
| Rwork | 0.233 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.316 |
| Data reduction software | SAINT |
| Data scaling software | SAINT (V7.51A) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.380 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.064 | 0.100 |
| Number of reflections | 17583 | |
| <I/σ(I)> | 38.84 | 15.41 |
| Completeness [%] | 99.9 | |
| Redundancy | 11.3 | 4.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 25% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 8.0, vapor diffusion, hanging drop, temperature 277K |
