3D4C

ZP-N domain of mammalian sperm receptor ZP3 (crystal form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-06
Detector	MAR CCD 165 mm
Wavelength(s)	0.95350
Spacegroup name	I 2 2 2
Unit cell lengths	93.040, 95.120, 141.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.500 - 2.900
R-factor	0.1922
Rwork	0.190
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ENSEMBLE DERIVED FROM PDB ENTRIES 1MG1 (chain A) 1NMU (chain C) 1T0K (chain A) 1YTV (chain B) 3MBP (chain A) 4MBP (chain A)
RMSD bond length	0.008
RMSD bond angle	0.640
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.500	3.080
High resolution limit [Å]	2.900	2.900
Number of reflections	14196
<I/σ(I)>	33.1	3.8
Completeness [%]	99.3	100
Redundancy	11.1	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Sample: 15mg/ml protein in 0.05M sodium chloride, 0.01M tris-HCL, PH7.2, 0.001M maltose. Reservoir: 1.0M sodium acetate, 0,1M sodium HEPES, PH7.5, 0.05M cadmium sulfate. Sample to reservoir ratio in drop: 1:1, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K