3D40
Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with diphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.38079 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 88.361, 88.361, 79.046 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.490 - 1.530 |
| R-factor | 0.16908 |
| Rwork | 0.169 |
| R-free | 0.19801 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | model of SeMet FomA form based on MAD data |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.738 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.580 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Number of reflections | 52986 | |
| <I/σ(I)> | 34.8 | 2.9 |
| Completeness [%] | 97.9 | 85.3 |
| Redundancy | 5.2 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 11% PEG 3350, 0.1 M tri-ammonium citrate, 0.1 M MES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
