3D3Z
Crystal structure of Actibind a T2 RNase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 78.279, 78.279, 103.968 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.630 - 1.700 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bol |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.647 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.200 | 0.830 |
| Number of reflections | 41379 | |
| <I/σ(I)> | 14.8 | 0.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.9 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 20% PEG 3350, 0.2 M ammonium dihydrogen phosphate solution at room temperature (293 K), pH 7.0, VAPOR DIFFUSION, pH 7.00 |
