3D3Q
Crystal structure of tRNA delta(2)-isopentenylpyrophosphate transferase (SE0981) from Staphylococcus epidermidis. Northeast Structural Genomics Consortium target SeR100
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97777 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.622, 84.480, 75.397 |
| Unit cell angles | 90.00, 102.87, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.700 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.094 | 0.341 |
| Number of reflections | 38850 | |
| <I/σ(I)> | 11.17 | 1.76 |
| Completeness [%] | 89.7 | 81.7 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.15 | 292 | 100 mM MES pH 6.15, 18% PEG 3350, 100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
