3D3M
The Crystal Structure of the C-terminal region of Death Associated Protein 5(DAP5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.97873, 0.91840 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.059, 45.734, 63.357 |
| Unit cell angles | 90.00, 103.81, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.25900 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.152 |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.068 | 0.330 |
| Number of reflections | 27035 | |
| <I/σ(I)> | 47.8 | 5.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under oil | 6 | 293 | 100mM MMT (D,L Maleic acid, MES and Tris) pH=6, and 20% PEG 1,500, Microbatch under oil, temperature 293K |
