3D3C
Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-09-07 |
| Detector | PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 113.000, 113.000, 267.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.22078 |
| Rwork | 0.218 |
| R-free | 0.27988 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 3D3B |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.141 | 0.314 |
| Number of reflections | 26358 | |
| <I/σ(I)> | 16.2 | 8.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.7 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | 0.1 M Tris-HCl, pH 7.1, 0.2 M ammonium sulfate, 25 % PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
