3D3A
Crystal structure of a beta-galactosidase from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 43 |
| Unit cell lengths | 117.017, 117.017, 59.711 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.460 - 2.150 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.122 | 0.293 |
| Number of reflections | 42306 | |
| <I/σ(I)> | 11.9 | 1 |
| Completeness [%] | 92.9 | 57.6 |
| Redundancy | 4.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 30% PEG 1500, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
