3D37
The crystal structure of the tail protein from Neisseria meningitidis MC58
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 137.460, 137.460, 137.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.130 - 2.100 |
| R-factor | 0.22219 |
| Rwork | 0.220 |
| R-free | 0.27346 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.817 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.130 | 2.155 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.113 | 0.570 |
| Number of reflections | 47918 | |
| <I/σ(I)> | 38.89 | 2.39 |
| Completeness [%] | 99.8 | 99.57 |
| Redundancy | 28.5 | 20.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M Potassium iodide, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
