3D32
Complex of GABA(A) receptor-associated protein (GABARAP) with a synthetic peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 129.287, 35.415, 58.209 |
Unit cell angles | 90.00, 100.59, 90.00 |
Refinement procedure
Resolution | 27.170 - 1.300 |
R-factor | 0.178 |
Rwork | 0.177 |
R-free | 0.19500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gnu |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.2) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.500 | 1.370 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.041 | 0.320 |
Number of reflections | 60022 | |
<I/σ(I)> | 16.3 | 2.7 |
Completeness [%] | 93.9 | 92.3 |
Redundancy | 2.1 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.9 | 285 | 30 % (w/v) PEG3350 800 mM NaCl 50 mM MES, pH 5.9, VAPOR DIFFUSION, temperature 285K |