3D30
Structure of an expansin like protein from Bacillus Subtilis at 1.9A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979782 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 143.780, 143.780, 143.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.400 - 1.900 |
| R-factor | 0.1646 |
| Rwork | 0.163 |
| R-free | 0.19067 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bh0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.517 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.400 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 38981 | |
| <I/σ(I)> | 16.1 | 2 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 14.9 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | 4M Na formate, 0.1M NaAc, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
